Flow-through polymerase chain reaction inside a seamless 3D helical microreactor fabricated utilizing a silicone tube and a paraffin mold.

نویسندگان

  • Wenming Wu
  • Kieu The Loan Trinh
  • Nae Yoon Lee
چکیده

We introduce a new strategy for fabricating a seamless three-dimensional (3D) helical microreactor utilizing a silicone tube and a paraffin mold. With this method, various shapes and sizes of 3D helical microreactors were fabricated, and a complicated and laborious photolithographic process, or 3D printing, was eliminated. With dramatically enhanced portability at a significantly reduced fabrication cost, such a device can be considered to be the simplest microreactor, developed to date, for performing the flow-through polymerase chain reaction (PCR).

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عنوان ژورنال:
  • The Analyst

دوره 140 5  شماره 

صفحات  -

تاریخ انتشار 2015